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1. Ninhydrin :

Ninhydrin, is a chemical used to detect ammonia or primary and secondary amines. When reacting with these free amines, a deep blue or purple color is evolved. Ninhydrin is most commonly used to detect fingerprints, as amines left over from peptides and proteins (terminal amines or lysine residues) sloughed off in fingerprints react with Ninhydrin.

Ninhydrin can also be used to monitor deprotection in solid phase peptide synthesis. When the growing peptide chain is deprotected, a ninhydrin test yields blue. If the next peptide residue is coupled then the test is colorless or yellow.
Ninhydrin is also used in amino acid analysis of proteins: Most of the amino acids are hydrolyzed and reacted with ninhydrin except Proline; Also, certain amino acid chains are degraded. Therefore, separate analysis is required for identifying such amino acids that either react differently or don't react at all with ninhydrin. The rest of the amino acids are then quantified colorimetrically after separation by chromatography.

A solution suspected of containing the ammonium ion can be tested by ninhydrin by dotting it onto a solid support (such as silica gel); treatment with ninhydrin should result in a dramatic purple color if the solution contains this species.(click here)

Ninhydrin
General
 Systematic name  Triketohydrindane hydrate
 Chemical formula  C9H6O4
 Appearance  White pale yellow crystals
 CAS number  [485-47-2]
 EINECS No  207-618-1


2. MERCURIC SULFATE

3. MERCURIC CHLORIDE

4. MERCURIC NITRATE

5. MERCURIC OXIDE